700044

antibody from Invitrogen Antibodies

Targeting: PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1

Western blot

Antibody data

Submit

Validation data

Product number: 700044 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-PLCG1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (C-p1p2-39H16L83)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with bovine, mouse, rat, Xenopus and zebrafish based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: C-p1p2-39H16L83
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PLCG1 protein structure - 700044 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-PLCG1 (Tyr783) Antibody (C-p1p2-39H16L83), Recombinant Rabbit Monoclonal (Product # 700044) at 2 µg/mL in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel f represents cells treated with antagonist, Afatinib (1 µM for 6 hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-PLCG2 (Tyr1217) staining. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-PLCG1 (Tyr783) Antibody (C-p1p2-39H16L83), Recombinant Rabbit Monoclonal (Product # 700044) at 2 µg/mL in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel f represents cells treated with antagonist, Afatinib (1 µM for 6 hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-PLCG2 (Tyr1217) staining. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.