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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX32238 - Provider product page
- Provider
- GeneTex
- Product name
- PLCG1 (phospho Tyr1253) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Netrin-1/DCC-mediated PLCĪ³1 activation is required for axon guidance and brain structure development.
Kang DS, Yang YR, Lee C, Park B, Park KI, Seo JK, Seo YK, Cho H, Lucio C, Suh PG
EMBO reports 2018 Nov;19(11)
EMBO reports 2018 Nov;19(11)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of PLC gamma 1 (pY1253) expression in MCF7 EGF-treated (A); A431 (B); mouse brain (C); mouse liver (D); H9C2 EGF-treated (E) whole cell lysates.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PLC gamma 1 (pY1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
