AM20220PU-N

antibody from OriGene

Targeting: PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20220PU-N

Provider

OriGene

Product name

Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) mouse monoclonal antibody, clone 2D5, Purified

Antibody type

Monoclonal

Description

Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) mouse monoclonal antibody, clone 2D5, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

PLCG1

Isotype

IgG

Antibody clone number

2D5

Vial size

100 µg

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous Phospholipase C gamma: Whole cell lysates of EGF stimulated serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM20220PU-N PLCG antibody (Clone 2D5) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2

Validation comment

WB