LS-C801045

antibody from LSBio

Targeting: PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C801045

Provider

LSBio

Product name

PLCG1 Antibody (phospho-Tyr771) LS-C801045

Antibody type

Polyclonal

Description

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.