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LearnAM20220PU-N
antibody from Acris Antibodies GmbH
Targeting: PLCG1
NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AM20220PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20220PU-N, RRID:AB_10756929
- Product name
- anti PLCG (970-990) (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 of PLCgamma (Phospholipase C gamma).
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D5
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection of endogenous Phospholipase C gamma: Whole cell lysates of EGF stimulated serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM20220PU-N PLCG antibody (Clone 2D5) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5Lane 5: HaCaTLane 6: PC3Lane 7: HeLaLane 8: HepG2
