Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS4866 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PLC ÎÛ2 (phospho Y1217) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PLC ÎÛ2 around the phosphorylation site of Tyrosine 1217.
- Description
- Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ÉÇ1, PLC ÉÇ2, PLC ÉÇ3, PLC ÉÇ4, PLCÎÛ1, PLCÎÛ2, PLC Ñ´1, PLC Ñ´2 and PLCe. PLCÑ´ exists as four different isoforms. PLC Ñ´1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC Ñ´1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and Ï«1B-adrenoreceptor signaling. Mouse PLC Ñ´1 is highly expressed in brain, heart, lung and testis. PLC Ñ´ is abnormally accumulated in autopsied brains with Alzheimer£ªs disease (AD), suggesting that it may play a role in the pathology of AD. PLC Ñ´2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC Ñ´2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC Ñ´2 expression may be a possible marker of gastric malignant transformation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of p-PLC ¦Ã2 (Y1217) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins)Lane2:sp2/0 cell lysate treated with EGF(0.1ng/ML,30mins)Lane3:H9C2 cell lysate treated with EGF(0.1ng/ML,30mins)