Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS64003 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PLC ÎÛ2 (phospho Y759) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human PLC ÎÛ2 around the phosphorylation site of Tyrosine 759.
- Description
- Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptormediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol fromphosphatidylinositol 4,5-bisphosphate.There are manymammalian PLC isozymes, including PLC ÉÇ1, PLC ÉÇ2, PLC ÉÇ3, PLC ÉÇ4, PLC ÎÛ1, PLC ÎÛ2, PLC Ñ´1, PLC Ñ´2 and PLC ÷. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC ÎÛ2 associateswith several tyrosine-phosphorylated proteins(Syk, SLP-76,Lyn, linker for activation of T cells(LAT) and the FcR ÎÛ chain),which bind to its C-terminal SH2 domain. PLC ÎÛ1 associateswith Syk in B cells, but PLC ÎÛ2 does not associatewith Sykin platelets. The C-terminal SH2 domain isinvolved in the regulation of PLC ÎÛ2. In addition, Btkcan induce PLC ÎÛ2 tyrosine phosphorylation and initiate calcium moblization in CD72- stimulated B lymphocytes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PLC ¦Ã2 (Y759) pAb at 1:500 dilutionLane1:Hela whole cell lysate Lane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate