Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36722 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PLCG2 (Tyr753) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 148 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PLCG2 (Tyr753) in Lane 1: HEK293T whole cell lysate, Lane 2: HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate, Lane 3: HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate, Lane 4: HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate, Lane 5: HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate. Samples were incubated with Phospho-PLCG2 (Tyr753) polyclonal antibody (Product # PA5-36722) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PLCG2 (Tyr753) in Lane 1: HEK293T whole cell lysate, Lane 2: HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate, Lane 3: HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate, Lane 4: HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate, Lane 5: HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate. Samples were incubated with Phospho-PLCG2 (Tyr753) polyclonal antibody (Product # PA5-36722) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PLC gamma-2 pTyr753 using Phospho-PLC gamma-2 pTyr753 polyclonal antibody (Product # PA5-36722) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Raw264.7 cell lysate treated with TNF-alpha, Lane 3: Mouse kidney tissue lysate, Lane 4: Rat kidney tissue lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-PLCG2 (Tyr753) in paraffin-embedded human colon cancer tissue. Samples were incubated with Phospho-PLCG2 (Tyr753) polyclonal antibody (Product # PA5-36722).