H00005336-D01P

antibody from Abnova Corporation

Targeting: PLCG2

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005336-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005336-D01P, RRID:AB_1710962

Product name

PLCG2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PLCG2 protein.

Antigen sequence

MSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRK
STPERRTVQVIMETRQVAWSKTADKIEGFLDIMEI
KEIRPGKNSKDFERAKAVRQKEDCCFTILYGTQFV
LSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPT
IIESWLRKQIYSVDQTRRNSISLRELKTILPLINF
KVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLM
FEQQKSILDEFKKDSSVFILGNTDRPDASAVYLRD
FQRFLIHEQQEHWAQDLNKVRERMTKFIDDTMRET
AEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMN
NPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLR
MGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDV
VQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKA
FKEVFGDLLLTKPTEASADQLPSPSQLREKIIIKH
KKLGPRGDVDVNMEDKKDEHKQQGELYMWDSIDQK
WTRHYCAIADAKLSFSDDIEQTMEEEVPQDIPPTE
LHFGEKWFHKKVEKRTSAEKLLQEYCMETGGKDGT
FLVRESETFPNDYTLSFWRSGRVQHCRIRSTMEGG
TLKYYLTDNLTFSSIYALIQHYRETHLRCAEFELR
LTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPR
DGAFLIRKREGSDSYAITFRARGKVKHCRINRDGR
HFVLGTSAYFESLVELVSYYEKHSLYRKMRLRYPV
TPELLERYNMERDINSLYDVSRMYVDPSEINPSMP
QRTVKALYDYKAKRSDELSFCRGALIHNVSKEPGG
WWKGDYGTRIQQYFPSNYVEDISTADFEELEKQII
EDNPLGSLCRGILDLNTYNVVKAPQGKNQKSFVFI
LEPKQQGYPPVEFATDRVEELFEWFQSIREITWKI
DTKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKD
NLENPDFREIRSFVETKADSIIRQKPVDLLKYNQK
GLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQ
TADKYMQMNHALFSLNGRTGYVLQPESMRTEKYDP
MPPESQRKILMTLTVKVLGARHLPKLGRSIACPFV
EVEICGAEYDNNKFKTTVVNDNGLSPIWAPTQEKV
TFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPI
KAVKSGFRSVPLKNGYSEDIELASLLVFCEMRPVL
ESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRN
ANRDALVKEFSVNENQLQLYQEKCNKRLREKRVSN
SKFYS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PLCG2 expression in transfected 293T cell line (H00005336-T02) by PLCG2 MaxPab polyclonal antibody.Lane 1: PLCG2 transfected lysate(147.90 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PLCG2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PLCG2 expression in Jurkat.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PLCG2 and PLCG1. HeLa cells were stained with anti-PLCG2 rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)