Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B130 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B130, RRID:AB_2165760
- Product name
- IHC-plus™ PPARG / PPAR Gamma Antibody LS-B130
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PPAR2 Antibody - Western Blot. Western blot of affinity purified anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.