LS-C209422

antibody from LSBio

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C209422

Provider

LSBio

Product name

PPARG / PPAR Gamma Antibody (phospho-Ser273) LS-C209422

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate