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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX60365 - Provider product page
- Provider
- GeneTex
- Product name
- PPAR gamma antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of 293T cells transfected with pNTAP-PPARG (lane 1) and non-transfected cells (lane 2) using PPARG antibody diluted 1:2,000 in TBS-Tween containing 3% skimmed milk.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of PPARG antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with the nuclear extract of mouse macrophage (derived from bone marrow) and either 1 £gg of PPARG antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to PDK4 PPAR response element (RE).
