HPA063663

antibody from Atlas Antibodies

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA063663

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA063663, RRID:AB_2685081

Product name

Anti-PPARG

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKP
HEEPSNSLMAIECRVC


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines RT-4 and U-251MG using Anti-PPARG antibody. Corresponding PPARG RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line RT4 shows localization to nucleus.

Sample type

HUMAN