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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11248 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11248, RRID:AB_1710913
- Product name
- PPARG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PPARG.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes.
Bacillus Calmette-Guérin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells.
The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts.
De Pascale MC, Bassi AM, Patrone V, Villacorta L, Azzi A, Zingg JM
Archives of biochemistry and biophysics 2006 Mar 15;447(2):97-106
Archives of biochemistry and biophysics 2006 Mar 15;447(2):97-106
Bacillus Calmette-Guérin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells.
Lodillinsky C, Umerez MS, Jasnis MA, Casabé A, Sandes E, Eiján AM
International journal of molecular medicine 2006 Feb;17(2):269-73
International journal of molecular medicine 2006 Feb;17(2):269-73
The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts.
Schild RL, Sonnenberg-Hirche CM, Schaiff WT, Bildirici I, Nelson DM, Sadovsky Y
Placenta 2006 Feb-Mar;27(2-3):191-9
Placenta 2006 Feb-Mar;27(2-3):191-9
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using PPARG polyclonal antibody (Cat # PAB11248) shows detection of PPARG protein in a mouse NIH/3T3 whole cell lysate (Lane 1 arrowhead).Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.Primary antibody was used at a 1 : 2,000 dilution in 5% BLOTTO PBS solution.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye.IgG™800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10.Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
