NBP2-59223

antibody from Novus Biologicals

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-59223

Provider

Novus Biologicals

Product name

Rabbit Polyclonal PPAR gamma/NR1C3 Antibody

Antibody type

Polyclonal

Description

Peptide affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

50 ug

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223] - The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (kDa) is shown on the left.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250