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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14095 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14095, RRID:AB_10553534
- Product name
- PPARG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PPARG.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 293T cells were analysed by Western blot using PPARG polyclonal antibody (Cat # PAB14095) diluted 1 : 500. Figure shows the result of 293T cells transfected with pNTAP-PPARG (Lane 2) or with an empty vector (Lane 1).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of PPARG polyclonal antibody (Cat # PAB14095). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 27,600.
- Validation comment
- ELISA
