Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB8316 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8316, RRID:AB_10616910
- Product name
- PPARG monoclonal antibody, clone 8D1H8F4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PPARG.
- Isotype
- IgG
- Antibody clone number
- 8D1H8F4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Peroxisome proliferator-activated receptor-gamma agonist is protective in podocyte injury-associated sclerosis.
Beyond peroxisome proliferator-activated receptor gamma signaling: the multi-facets of the antitumor effect of thiazolidinediones.
Transforming growth factor-beta inhibits CCAAT/enhancer-binding protein expression and PPARgamma activity in unloaded bone marrow stromal cells.
Yang HC, Ma LJ, Ma J, Fogo AB
Kidney international 2006 May;69(10):1756-64
Kidney international 2006 May;69(10):1756-64
Beyond peroxisome proliferator-activated receptor gamma signaling: the multi-facets of the antitumor effect of thiazolidinediones.
Weng JR, Chen CY, Pinzone JJ, Ringel MD, Chen CS
Endocrine-related cancer 2006 Jun;13(2):401-13
Endocrine-related cancer 2006 Jun;13(2):401-13
Transforming growth factor-beta inhibits CCAAT/enhancer-binding protein expression and PPARgamma activity in unloaded bone marrow stromal cells.
Ahdjoudj S, Kaabeche K, Holy X, Fromigué O, Modrowski D, Zérath E, Marie PJ
Experimental cell research 2005 Feb 1;303(1):138-47
Experimental cell research 2005 Feb 1;303(1):138-47
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using PPARG monoclonal antibody, clone 8D1H8F4 (Cat # MAB8316) against truncated PPARG recombinant protein (Lane 1) and K-562 cell lysate (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal analysis of A-431 cells using PPARG monoclonal antibody, clone 8D1H8F4 (Cat # MAB8316) showing nuclear staining.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human carcinoma and normal tissue, showing nuclear localization using PPARG monoclonal antibody, clone 8D1H8F4 (Cat # MAB8316) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)