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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6963 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPARG Antibody / PPAR gamma
- Antibody type
- Polyclonal
- Description
- This highly specific PPARG antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PPARG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) MCF7, 3) SK-O-V3 and 4) MDA-MB-453 cell lysate with PPARG antibody. Predicted molecular weight: 54-57 kDa, observed here at ~67 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with PPARG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with PPARG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPARG antibody.
