Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- R1491P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1491P, RRID:AB_1006261
- Product name
- anti PPAR-gamma (PPARG2) (N-term)
- Antibody type
- Polyclonal
- Antigen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2.PPAR gamma 2 is a 55 kDa protein.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot using affinity purified anti-PPARG2 antibody shows detection of PPARG2 protein in a Mouse 3T3 whole cell lysate (Lane 1 arrow head). Approximately 20 µg of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1/2,000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1/10,000 dilution of IRDye TM800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M). Other detection systems will yield similar results.