R1491P

antibody from Acris Antibodies GmbH

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1491P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1491P, RRID:AB_1006261

Product name

anti PPAR-gamma (PPARG2) (N-term)

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2.PPAR gamma 2 is a 55 kDa protein.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using affinity purified anti-PPARG2 antibody shows detection of PPARG2 protein in a Mouse 3T3 whole cell lysate (Lane 1 arrow head). Approximately 20 µg of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1/2,000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1/10,000 dilution of IRDye TM800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M). Other detection systems will yield similar results.