H00006777-M04

antibody from Abnova Corporation

Targeting: STAT5B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006777-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006777-M04, RRID:AB_733248

Product name

STAT5B monoclonal antibody (M04), clone 1C2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant STAT5B.

Antigen sequence

MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLS
QWIESQAWDSVDLDNPQENIKATQLLEGLVQELQK
KAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMEL
VRCIRHILYNEQRLVREANNGSSPAGSLADAMSQK
HLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQ
YQESLRIQAQFGPLAQLSPQERLSRETALQQKQVS
LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQT
IILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCE
KLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAE
VNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA
TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKN
ENTRK


Isotype

IgG

Antibody clone number

1C2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STAT5B monoclonal antibody (M04), clone 1C2 Western Blot analysis of STAT5B expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of STAT5B expression in transfected 293T cell line by STAT5B monoclonal antibody (M04), clone 1C2.Lane 1: STAT5B transfected lysate (Predicted MW: 44.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged STAT5B is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of STAT5B transfected lysate using anti-STAT5B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with STAT5B monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol