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- Antigen structure
- References [41]
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- Product number
- MA1-10293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD86 Monoclonal Antibody (BU63)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BU63
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references High-fluence low-power laser irradiation promotes odontogenesis and inflammation resolution in periodontitis by enhancing stem cell proliferation and differentiation.
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.
Human dendritic cell culture and bacterial infection.
HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation.
Human follicular dendritic cells promote the APC capability of B cells by enhancing CD86 expression levels.
Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.
Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.
Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef.
The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells.
HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis.
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95.
A novel human B cell subpopulation representing the initial germinal center population to express AID.
Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.
Human monocyte-derived dendritic cells are a source of several complement proteins.
Expression of CD45 and proliferative response to CD3 as suitable classification markers of patients with gastric adenocarcinoma.
Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.
Profiling dendritic cell maturation with dedicated microarrays.
Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation.
Bacterial ghosts as novel efficient targeting vehicles for DNA delivery to the human monocyte-derived dendritic cells.
Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV-infected immature dendritic cells.
Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.
Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression.
Oxidized phospholipids negatively regulate dendritic cell maturation induced by TLRs and CD40.
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
CD63 as an activation-linked T cell costimulatory element.
CD63 as an activation-linked T cell costimulatory element.
Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines.
Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines.
White monkey syndrome in infant baboons (Papio species).
Intrinsic defects explain altered proliferative responses of T lymphocytes and HVS-derived T-cell lines in gastric adenocarcinoma.
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning.
Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.
Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.
Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
Hou T, Li S, Zhang G, Li Y
International journal of molecular medicine 2018 Oct;42(4):2107-2119
International journal of molecular medicine 2018 Oct;42(4):2107-2119
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
Mytych J, Romerowicz-Misielak M, Koziorowski M
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.
Babu R, Brown A
Retrovirology 2013 Dec 16;10:155
Retrovirology 2013 Dec 16;10:155
Human dendritic cell culture and bacterial infection.
Jones HE, Klein N, Dixon GL
Methods in molecular biology (Clifton, N.J.) 2012;799:217-35
Methods in molecular biology (Clifton, N.J.) 2012;799:217-35
HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation.
St Gelais C, Coleman CM, Wang JH, Wu L
PloS one 2012;7(3):e34521
PloS one 2012;7(3):e34521
Human follicular dendritic cells promote the APC capability of B cells by enhancing CD86 expression levels.
Kim J, Kim YM, Jeoung DI, Choe J
Cellular immunology 2012;273(2):109-14
Cellular immunology 2012;273(2):109-14
Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.
Garbe K, Bratke K, Wagner S, Virchow JC, Lommatzsch M
Human immunology 2012 May;73(5):493-7
Human immunology 2012 May;73(5):493-7
Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.
Morbach H, Wiegering V, Richl P, Schwarz T, Suffa N, Eichhorn EM, Eyrich M, Girschick HJ
Arthritis and rheumatism 2011 Nov;63(11):3458-66
Arthritis and rheumatism 2011 Nov;63(11):3458-66
Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef.
Coleman CM, Spearman P, Wu L
Retrovirology 2011 Apr 19;8:26
Retrovirology 2011 Apr 19;8:26
The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells.
Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger GJ, Stuhlmeier KM, Sachet M, Seipelt J, Stöckl J
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4440-8
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4440-8
HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis.
Kuhne M, Erben U, Schulze-Tanzil G, Köhler D, Wu P, Richter FJ, John T, Radbruch A, Sieper J, Appel H
Arthritis and rheumatism 2009 Jun;60(6):1635-46
Arthritis and rheumatism 2009 Jun;60(6):1635-46
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Stephens TA, Nikoopour E, Rider BJ, Leon-Ponte M, Chau TA, Mikolajczak S, Chaturvedi P, Lee-Chan E, Flavell RA, Haeryfar SM, Madrenas J, Singh B
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Stephens TA, Nikoopour E, Rider BJ, Leon-Ponte M, Chau TA, Mikolajczak S, Chaturvedi P, Lee-Chan E, Flavell RA, Haeryfar SM, Madrenas J, Singh B
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95.
Jacobi AM, Reiter K, Mackay M, Aranow C, Hiepe F, Radbruch A, Hansen A, Burmester GR, Diamond B, Lipsky PE, Dörner T
Arthritis and rheumatism 2008 Jun;58(6):1762-73
Arthritis and rheumatism 2008 Jun;58(6):1762-73
A novel human B cell subpopulation representing the initial germinal center population to express AID.
Kolar GR, Mehta D, Pelayo R, Capra JD
Blood 2007 Mar 15;109(6):2545-52
Blood 2007 Mar 15;109(6):2545-52
Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.
Summers KL, Marleau AM, Mahon JL, McManus R, Hramiak I, Singh B
Clinical immunology (Orlando, Fla.) 2006 Oct;121(1):81-9
Clinical immunology (Orlando, Fla.) 2006 Oct;121(1):81-9
Human monocyte-derived dendritic cells are a source of several complement proteins.
Reis ES, Barbuto JA, Isaac L
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 May;55(5):179-84
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 May;55(5):179-84
Expression of CD45 and proliferative response to CD3 as suitable classification markers of patients with gastric adenocarcinoma.
López-Santalla M, Valeri AP, Pérez-Blas M, Aguilera-Montilla N, Gutiérrez A, Lasa I, Mugüerza JM, Martín J, García-Sancho L, Granell J, Martín-Villa JM
Cancer immunology, immunotherapy : CII 2006 Jun;55(6):744-8
Cancer immunology, immunotherapy : CII 2006 Jun;55(6):744-8
Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.
Sandilands GP, McCrae J, Hill K, Perry M, Baxter D
Immunology 2006 Dec;119(4):562-71
Immunology 2006 Dec;119(4):562-71
Profiling dendritic cell maturation with dedicated microarrays.
McIlroy D, Tanguy-Royer S, Le Meur N, Guisle I, Royer PJ, Léger J, Meflah K, Grégoire M
Journal of leukocyte biology 2005 Sep;78(3):794-803
Journal of leukocyte biology 2005 Sep;78(3):794-803
Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation.
Sandilands GP, Ahmed Z, Perry N, Davison M, Lupton A, Young B
Immunology 2005 Mar;114(3):354-68
Immunology 2005 Mar;114(3):354-68
Bacterial ghosts as novel efficient targeting vehicles for DNA delivery to the human monocyte-derived dendritic cells.
Kudela P, Paukner S, Mayr UB, Cholujova D, Schwarczova Z, Sedlak J, Bizik J, Lubitz W
Journal of immunotherapy (Hagerstown, Md. : 1997) 2005 Mar-Apr;28(2):136-43
Journal of immunotherapy (Hagerstown, Md. : 1997) 2005 Mar-Apr;28(2):136-43
Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV-infected immature dendritic cells.
Lozza L, Lilleri D, Percivalle E, Fornara C, Comolli G, Revello MG, Gerna G
European journal of immunology 2005 Jun;35(6):1795-804
European journal of immunology 2005 Jun;35(6):1795-804
Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.
Game DS, Rogers NJ, Lechler RI
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2005 Jul;5(7):1614-25
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2005 Jul;5(7):1614-25
Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression.
Kirchberger S, Majdic O, Steinberger P, Blüml S, Pfistershammer K, Zlabinger G, Deszcz L, Kuechler E, Knapp W, Stöckl J
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 15;175(2):1145-52
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 15;175(2):1145-52
Oxidized phospholipids negatively regulate dendritic cell maturation induced by TLRs and CD40.
Blüml S, Kirchberger S, Bochkov VN, Krönke G, Stuhlmeier K, Majdic O, Zlabinger GJ, Knapp W, Binder BR, Stöckl J, Leitinger N
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):501-8
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):501-8
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Zingoni A, Sornasse T, Cocks BG, Tanaka Y, Santoni A, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Zingoni A, Sornasse T, Cocks BG, Tanaka Y, Santoni A, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
CD63 as an activation-linked T cell costimulatory element.
Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6000-8
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6000-8
CD63 as an activation-linked T cell costimulatory element.
Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6000-8
Journal of immunology (Baltimore, Md. : 1950) 2004 Nov 15;173(10):6000-8
Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines.
Aguilera-Montilla N, Pérez-Blas M, Valeri AP, López-Santalla M, Rodríguez-Juan C, Mencía A, Castellano G, Manzano ML, Casis B, Sánchez F, Martín-Villa JM
Scandinavian journal of gastroenterology 2004 Dec;39(12):1236-42
Scandinavian journal of gastroenterology 2004 Dec;39(12):1236-42
Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines.
Aguilera-Montilla N, Pérez-Blas M, Valeri AP, López-Santalla M, Rodríguez-Juan C, Mencía A, Castellano G, Manzano ML, Casis B, Sánchez F, Martín-Villa JM
Scandinavian journal of gastroenterology 2004 Dec;39(12):1236-42
Scandinavian journal of gastroenterology 2004 Dec;39(12):1236-42
White monkey syndrome in infant baboons (Papio species).
Frost PA, Hubbard GB, Dammann MJ, Snider CL, Moore CM, Hodara VL, Giavedoni LD, Rohwer R, Mahaney MC, Butler TM, Cummins LB, McDonald TJ, Nathanielsz PW, Schlabritz-Loutsevitch NE
Journal of medical primatology 2004 Aug;33(4):197-213
Journal of medical primatology 2004 Aug;33(4):197-213
Intrinsic defects explain altered proliferative responses of T lymphocytes and HVS-derived T-cell lines in gastric adenocarcinoma.
Valeri AP, Pérez-Blas M, Gutiérrez A, López-Santalla M, Aguilera N, Rodríguez-Juan C, Sala-Silveira L, Martín J, Lasa I, Mugüerza JM, López A, García-Sancho L, Granell J, Martín-Villa JM
Cancer immunology, immunotherapy : CII 2003 Nov;52(11):708-14
Cancer immunology, immunotherapy : CII 2003 Nov;52(11):708-14
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
Selenko-Gebauer N, Majdic O, Szekeres A, Höfler G, Guthann E, Korthäuer U, Zlabinger G, Steinberger P, Pickl WF, Stockinger H, Knapp W, Stöckl J
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3637-44
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3637-44
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
Selenko-Gebauer N, Majdic O, Szekeres A, Höfler G, Guthann E, Korthäuer U, Zlabinger G, Steinberger P, Pickl WF, Stockinger H, Knapp W, Stöckl J
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3637-44
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3637-44
Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning.
Steinberger P, Szekeres A, Wille S, Stöckl J, Selenko N, Prager E, Staffler G, Madic O, Stockinger H, Knapp W
Journal of leukocyte biology 2002 Jan;71(1):133-40
Journal of leukocyte biology 2002 Jan;71(1):133-40
Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.
McDowell MA, Marovich M, Lira R, Braun M, Sacks D
Infection and immunity 2002 Aug;70(8):3994-4001
Infection and immunity 2002 Aug;70(8):3994-4001
Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.
McDowell MA, Marovich M, Lira R, Braun M, Sacks D
Infection and immunity 2002 Aug;70(8):3994-4001
Infection and immunity 2002 Aug;70(8):3994-4001
Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
Berg LP, James MJ, Alvarez-Iglesias M, Glennie S, Lechler RI, Marelli-Berg FM
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 1;168(7):3227-34
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 1;168(7):3227-34
Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
Berg LP, James MJ, Alvarez-Iglesias M, Glennie S, Lechler RI, Marelli-Berg FM
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 1;168(7):3227-34
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 1;168(7):3227-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of human Raji cells (left panel) and mouse spenocytes (right panel) using CD86 monoclonal antibody (Product # MA1-10293) at 0.5µg x 10^6 cells in 100µL. CD86 (green) was detected using a DyLight-488 Donkey anti-Mouse IgG (H+L) cross-adsorbed secondary antibody (Product # A-21202) at 1:200. The negative control (blue) represents Mouse IgG Isotype Control (Product # MA5-14453). Results show positive staining in human cells with no cross-reactivity to mouse. Data provided courtesy of Antibody Resource.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD86 (BU63) purified Monoclonal antibody (Product # MA1-10293) (concentration in sample 3 µg/mL) GAM APC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD86 (BU63) purified Monoclonal antibody (Product # MA1-10293) (concentration in sample 3 µg/mL) GAM APC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 HF-LPLI promoted neutrophil apoptosis and MPhi reprogramming, and inhibited the release of inflammatory factors. (A) Apoptosis of HF-LPLI-treated neutrophils was detected by flow cytometry. (B) Apoptosis activation in neutrophils, detected by caspase-3 activity assay. (C) CD86 and CD163 immunofluo-rescent staining using red and green fluorophores, respectively, and nuclei stained with DAPI (scale bar, 100 mu m). (D) Bar graph of CD86- and CD163-positive MPhi percentage by immunofluorescent staining. (E) NO concentration examined by DAF-FM DA. (F) Concentration of inflammatory factors, including TNF-alpha, IL-6, IL-10 and TGF-beta, determined by ELISA. The results are representative of 10 independent experiments, and are expressed as the mean +- standard deviation. **P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 HF-LPLI reduced inflammatory cell infiltration in a periodontitis rat model. (A) Immunofluorescence analysis demonstrating green-stained CD11b neutrophils and red-stained CD86 macrophages (scale bar, 100 mu m). (B) Bar graph of inflammatory cell number in the local periodontitis tissue in the rat model, examined by immunofluorescence. *P
