Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA1-10296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD86 Monoclonal Antibody (BU63), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- BU63
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model.
Development of a skin- and neuro-attenuated live vaccine for varicella.
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.
Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Morell M, Varela N, Castillejo-López C, Coppard C, Luque MJ, Wu YY, Martín-Morales N, Pérez-Cózar F, Gómez-Hernández G, Kumar R, O'Valle F, Alarcón-Riquelme ME, Marañón C
EBioMedicine 2022 Feb;76:103808
EBioMedicine 2022 Feb;76:103808
Development of a skin- and neuro-attenuated live vaccine for varicella.
Wang W, Pan D, Fu W, Ye X, Han J, Yang L, Jia J, Liu J, Zhu R, Zhang Y, Liu C, Ye J, Selariu A, Que Y, Zhao Q, Wu T, Li Y, Zhang J, Cheng T, Zhu H, Xia N
Nature communications 2022 Feb 11;13(1):824
Nature communications 2022 Feb 11;13(1):824
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Stephens TA, Nikoopour E, Rider BJ, Leon-Ponte M, Chau TA, Mikolajczak S, Chaturvedi P, Lee-Chan E, Flavell RA, Haeryfar SM, Madrenas J, Singh B
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.
Stephens TA, Nikoopour E, Rider BJ, Leon-Ponte M, Chau TA, Mikolajczak S, Chaturvedi P, Lee-Chan E, Flavell RA, Haeryfar SM, Madrenas J, Singh B
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):6859-71
Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.
Summers KL, Marleau AM, Mahon JL, McManus R, Hramiak I, Singh B
Clinical immunology (Orlando, Fla.) 2006 Oct;121(1):81-9
Clinical immunology (Orlando, Fla.) 2006 Oct;121(1):81-9
Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.
Game DS, Rogers NJ, Lechler RI
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2005 Jul;5(7):1614-25
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2005 Jul;5(7):1614-25
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD86 (BU63) PE Monoclonal antibody (Product # MA1-10296).
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 v7D induces functional activation of human DCs in vitro. Human iDCs were generated from CD14 + monocytes purified from PBMCs, and were treated with rOka, v7D, vOka (MOI = 0.01) or mock-infected MRC-5 cell lysate as a control. a , b Representative flow cytometry data and summary of VZV-gE expression on iDCs at 3 dpi. rOka-infected iDCs were stained with an isotype antibody (iso) as a control. Numbers on the right side of each graph in ( a ) represent the percent of VZV-gE-positive cells. c Flow cytometry analysis of expression of the cell-surface costimulatory markers CD40, CD80, CD83 and CD86 on DCs at 3 dpi. Representative flow cytometry data are shown in Supplementary Fig. 5 . d Analysis of percent lysis of DCs compared to that of MRC-5 cells after inoculation with v7D and vOka (MOI = 0.01) over five days. e Cytokine/chemokine analysis of DC culture supernatants. f Analysis of cell proliferation rates of the purified autologous CD4 + and CD8 + T cells after co-culture with antigen-pulsed DCs using the BrdU ELISA assay. g , h Representative flow cytometry data and summary data of CFSE dilution among CD4 + and CD8 + T cells after 5-day co-culture with antigen-pulsed DCs. i Analysis of IFN-gamma production in CD4 + and CD8 + T cells after 5 days of co-culture with antigen-pulsed DCs using an ELISPOT assay. The results are represented as averages +- the SD ( n = 3 per group). Asterisks denote a significant difference ( P < 0.05) compared to the mock controls as determine
- Conjugate
- Yellow dye