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Blocking/NeutralizingAntibody data
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- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-30148 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal B7-2/CD86 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression.
McLellan AD, Kapp M, Eggert A, Linden C, Bommhardt U, Bröcker EB, Kämmerer U, Kämpgen E
Blood 2002 Mar 15;99(6):2084-93
Blood 2002 Mar 15;99(6):2084-93
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Enhanced validation
- Submitted by
- Novus Biologicals (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Knockout Validated: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Rat Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-30148) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #HAF005). Specific band was detected for B7-2 at approximately 80 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD86 (B7-2) (filled histogram) followed by Anti-Rat IgG FITC.
