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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB13401 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat B7-2/CD86 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat B7-1, recombinant human B7-2, recombinant mouse (rm) B7-2, rmB7-H1, rmB7-H2, rmB7-H3, or rmPD-L is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_064466- Isotype
- IgG
- Antibody clone number
- 199622
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-2 Secretion Induced by B7-2/CD86 and Neutralization by Rat B7-2/CD86 Antibody. Recombinant Rat B7-2/CD86 Fc Chimera (Catalog # 1340-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Rat B7-2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7-2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND50 is typically 0.3-1.0 µg/mL.
