Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-47778-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal B7-2/CD86 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free [NBP2-47778] - Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free [NBP2-47778] - Human Melanoma stained with CD86 Monoclonal Antibody (C86/1146).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free [NBP2-47778] - A cell surface stain was performed on Daudi cells with B7-2/CD86 (C86/1146) antibody NBP2-47778 and a matched isotype control NBP2-27287. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free [NBP2-47778] - A cell surface stain was performed on Daudi cells with B7-2/CD86 (C86/1146) antibody NBP2-47778PCP and a matched isotype control NBP2-27287PCP. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Image using the PerCP format of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free [NBP2-47778] - Human PBMC's using CD86 Monoclonal Antibody (C86/1146).