Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Other assay [4]
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- Product number
- MHCD8601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD86 Monoclonal Antibody (BU63), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- BU63
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Baicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization.
Macrophages promote matrix protrusive and invasive function of breast cancer cells via MIP-1β dependent upregulation of MYO3A gene in breast cancer cells.
Induction of type I interferon signaling by Pseudomonas aeruginosa is diminished in cystic fibrosis epithelial cells.
Ca2+/calmodulin-dependent kinase kinase alpha is expressed by monocytic cells and regulates the activation profile.
The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Commitment of juvenile myelo-monocytic (JMML) leukemic cells to spontaneously differentiate into dendritic cells.
CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
Zhao X, Qu J, Liu X, Wang J, Ma X, Zhao X, Yang Q, Yan W, Zhao Z, Hui Y, Bai H, Zhang S
American journal of cancer research 2018;8(8):1528-1540
American journal of cancer research 2018;8(8):1528-1540
Macrophages promote matrix protrusive and invasive function of breast cancer cells via MIP-1β dependent upregulation of MYO3A gene in breast cancer cells.
Baghel KS, Tewari BN, Shrivastava R, Malik SA, Lone MU, Jain NK, Tripathi C, Kanchan RK, Dixit S, Singh K, Mitra K, Negi MP, Srivastava M, Misra S, Bhatt ML, Bhadauria S
Oncoimmunology 2016 Jul;5(7):e1196299
Oncoimmunology 2016 Jul;5(7):e1196299
Induction of type I interferon signaling by Pseudomonas aeruginosa is diminished in cystic fibrosis epithelial cells.
Parker D, Cohen TS, Alhede M, Harfenist BS, Martin FJ, Prince A
American journal of respiratory cell and molecular biology 2012 Jan;46(1):6-13
American journal of respiratory cell and molecular biology 2012 Jan;46(1):6-13
Ca2+/calmodulin-dependent kinase kinase alpha is expressed by monocytic cells and regulates the activation profile.
Guest CB, Deszo EL, Hartman ME, York JM, Kelley KW, Freund GG
PloS one 2008 Feb 13;3(2):e1606
PloS one 2008 Feb 13;3(2):e1606
The immunogenicity of human adipose-derived cells: temporal changes in vitro.
McIntosh K, Zvonic S, Garrett S, Mitchell JB, Floyd ZE, Hammill L, Kloster A, Di Halvorsen Y, Ting JP, Storms RW, Goh B, Kilroy G, Wu X, Gimble JM
Stem cells (Dayton, Ohio) 2006 May;24(5):1246-53
Stem cells (Dayton, Ohio) 2006 May;24(5):1246-53
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74
Journal of virology 2003 Jul;77(13):7563-74
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74
Journal of virology 2003 Jul;77(13):7563-74
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
Commitment of juvenile myelo-monocytic (JMML) leukemic cells to spontaneously differentiate into dendritic cells.
Longoni D, D'Amico G, Gaipa G, Bernasconi S, Vulcano M, Onnis P, Niemeyer CM, Allavena P, Biondi A
The hematology journal : the official journal of the European Haematology Association 2002;3(6):302-10
The hematology journal : the official journal of the European Haematology Association 2002;3(6):302-10
CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
Todisco E, Gaipa G, Biagi E, Bonamino M, Gramigna R, Introna M, Biondi A
Leukemia 2002 Oct;16(10):2046-54
Leukemia 2002 Oct;16(10):2046-54
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
Ng WF, Duggan PJ, Ponchel F, Matarese G, Lombardi G, Edwards AD, Isaacs JD, Lechler RI
Blood 2001 Nov 1;98(9):2736-44
Blood 2001 Nov 1;98(9):2736-44
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
Ng WF, Duggan PJ, Ponchel F, Matarese G, Lombardi G, Edwards AD, Isaacs JD, Lechler RI
Blood 2001 Nov 1;98(9):2736-44
Blood 2001 Nov 1;98(9):2736-44
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 The CaMKKalpha inhibitor STO-609 enhances PMA-dependent up-regulation of CD86 but blocks CD11b expression and CaMKKalpha nuclear localization. (A and B) U937 cells were pretreated with or without 5 ug/ml STO-609 for 6 h prior to activation with 100 nM PMA for 48 h, as indicated. Surface expression of CD11b and CD86 was quantified by flow cytometry using FITC-conjugated CD11b and PE-conjugated CD86 antibodies. Results significantly different from PMA at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM. (C) Cells were treated as in panel A and CaMKKalpha was quantified by Western analysis of nuclear lysates. Results are representative of three independent experiments. (D) U937 cells were treated as in panel A and ERK1/2 phosphorylation (ERK-p) and mass (ERK) were measured by Western analysis in whole cell lysates. Results are representative of three independent experiments.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Forskolin enhances PMA-dependent CD86 expression while inhibiting CD11b up-regulation and CaMKKalpha nuclear localization. (A and B) U937 cells were pretreated with or without 30 uM forskolin for 1 h prior to activation with 100 nM PMA for 48 h, as indicated. Surface expression of CD11b and CD86 was quantified by flow cytometry using FITC-conjugated CD11b and PE-conjugated CD86 antibodies. Results significantly different from PMA at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM. (C) Cells were treated as in panel A and CaMKKalpha was quantified by Western analysis of nuclear lysates. Results are representative of three independent experiments. (D) U937 cells were treated as in panel A and ERK1/2 phosphorylation (ERK-p) and mass (ERK) were measured by Western analysis in whole cell lysates. Results are representative of three independent experiments.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 The PKC inhibitor Bisindolylmaleimide inhibits PMA-dependent upregulation of CD86/CD11b expression. (A & B) U937 cells were pretreated with or without 1 uM bisindolylmaleimide for 15 min prior to activation with 100 nM PMA for 48 h, as indicated. Surface expression of CD11b and CD86 was quantified by flow cytometry using FITC-conjugated CD11b and PE-conjugated CD86 antibodies. Results significantly different from PMA at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0001606.g005 Figure 5 Inhibition of CaMKKalpha by siRNA enhances PMA-dependent up-regulation of CD86 but decreases CD11b expression. (A and B) Cells were treated with 0.25 ug of either CaMKKalpha siRNA or scramble siRNA control prior to stimulation with 100 nM PMA for 48 h. Surface expression of CD11b and CD86 was quantified by flow cytometry. Results significantly different from PMA at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM. (C) Cells were treated as in A and CaMKKalpha protein expression was quantified by intracellular flow. Results significantly different from control at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM.
- Conjugate
- Green dye