Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 16-0869-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD86 (B7-2) Monoclonal Antibody (IT2.2), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. IT2.2 has also been reported in blocking of CD86 in functional studies. Applications Tested: The IT2.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- IT2.2
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Pentraxin-3 regulates the inflammatory activity of macrophages.
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Endothelial microparticles interact with and support the proliferation of T cells.
TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells.
Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.
Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells.
B70 antigen is a second ligand for CTLA-4 and CD28.
Guo M, Xiao J, Sheng X, Zhang X, Tie Y, Wang L, Zhao L, Ji X
Frontiers in pharmacology 2018;9:464
Frontiers in pharmacology 2018;9:464
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Xiu W, Ma J, Lei T, Zhang M
Oncology letters 2018 Sep;16(3):2851-2856
Oncology letters 2018 Sep;16(3):2851-2856
Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.
Zhang F, Zhu Y, Fan G, Hu S
Oncology letters 2018 Oct;16(4):5034-5040
Oncology letters 2018 Oct;16(4):5034-5040
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Pentraxin-3 regulates the inflammatory activity of macrophages.
Shiraki A, Kotooka N, Komoda H, Hirase T, Oyama JI, Node K
Biochemistry and biophysics reports 2016 Mar;5:290-295
Biochemistry and biophysics reports 2016 Mar;5:290-295
MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.
Zhong Y, Yi C
Bioscience reports 2016 Aug;36(4)
Bioscience reports 2016 Aug;36(4)
Endothelial microparticles interact with and support the proliferation of T cells.
Wheway J, Latham SL, Combes V, Grau GE
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3378-87
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3378-87
TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells.
Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):7863-72
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):7863-72
Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.
Schierloh P, Yokobori N, Alemán M, Landoni V, Geffner L, Musella RM, Castagnino J, Baldini M, Abbate E, de la Barrera SS, Sasiain MC
Infection and immunity 2007 Nov;75(11):5325-37
Infection and immunity 2007 Nov;75(11):5325-37
Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
Kapsogeorgou EK, Moutsopoulos HM, Manoussakis MN
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3107-13
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3107-13
CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells.
Jeannin P, Delneste Y, Lecoanet-Henchoz S, Gauchat JF, Ellis J, Bonnefoy JY
The Journal of biological chemistry 1997 Jun 20;272(25):15613-9
The Journal of biological chemistry 1997 Jun 20;272(25):15613-9
B70 antigen is a second ligand for CTLA-4 and CD28.
Azuma M, Ito D, Yagita H, Okumura K, Phillips JH, Lanier LL, Somoza C
Nature 1993 Nov 4;366(6450):76-9
Nature 1993 Nov 4;366(6450):76-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD86 (B7-2) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.