A700-030

antibody from Invitrogen Antibodies

Targeting: CD86 B7-2, B7.2, CD28LG2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-030

Provider

Invitrogen Antibodies

Product name

CD86 Recombinant Rabbit Monoclonal Antibody (BLR030F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR030F

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 by western blot. Samples: Whole cell lysate (5, 15 or 50 µg) from HEK293T, HDLM-2, Jurkat, MJ, KG-1, Ramos, SUP-M2, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (Product # A700-030 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anit-Actin recombinant monoclonal antibody [BLR059F] (Product # A700-059).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (A700-030) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (A700-029). ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 by WB of HDLM-2, MJ [G11], SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, 293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (A700-030). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH (A300-641A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE HDLM-2 by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (Product # A700-030 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE HDLM-2 by ICC.Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (A700-030).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (Product # A700-030 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (Product # A700-030-T lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE seminoma by IHC.Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (A700-030).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 in FFPE tonsil by IHC.Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (A700-030).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 (shaded) in MJ[G11) cells by flow cytometry. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (Product #A700-030; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD86 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (Product # A700-030 lot 3) used for IP at 6 µL per reaction. CD86 was also immunoprecipitated by a previous lot of this antibody (lot A700-030-2) and rabbit anti-CD86 recombinant monoclonal antibody [BLR029F] (Product # A700-029). For blotting immunoprecipitated CD86 (Product # A700-030) was used at 1:1000. Chemiluminescence with an exposure time of 1 second.