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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- Ab095464 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD86 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human CD86 Antibody, Monoclonal (BU63), could be used for Flow and so on.Application:Flow: 1.0 μg/100w cellsProtein FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
B lymphoblastoid cell line- Antibody clone number
- BU63
- Vial size
- 100μg,10μg,1mg,50μg
- Concentration
- Lot by Lot
- Storage
- Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.
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Supportive validation
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- Aladdin Scientific (provider)
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- Experimental details
- Anti-CD86 antibody (Ab095464) - Flow Cytometry Flow Cytometry analysis of Raji cells labelling CD86 (red) with Anti-CD86 antibody (Ab095464) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
