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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-19913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DFFB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~40kDa. A suggested positive control is K562 cell lysate. PA5-19913 can be used with blocking peptide PEP-0039.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Prolonged mitotic arrest induces a caspase-dependent DNA damage response at telomeres that determines cell survival.
Hain KO, Colin DJ, Rastogi S, Allan LA, Clarke PR
Scientific reports 2016 May 27;6:26766
Scientific reports 2016 May 27;6:26766
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate using a DFF40 polyclonal antibody (Product # PA5-19913) at 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFFB Polyclonal Antibody (Product # PA5-19913) (I18) at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of DFF40 in Jurkat cells with DFFB Polyclonal Antibody (Product # PA5-19913) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of DFF40 in K562 cells with DFFB Polyclonal Antibody (Product # PA5-19913) at 20 µg/mL. Green: DFF40 Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Jurkat cells using a DFF40 polyclonal antibody (Product # PA5-19913) at a 5 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
