F55043

antibody from NSJ Bioreagents

Targeting: DFFB CAD, CPAN, DFF-40, DFF40

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55043

Provider

NSJ Bioreagents

Product name

DFFB Antibody / DNA fragmentation factor subunit beta

Antibody type

Polyclonal

Description

This highly specific DFFB antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the DFFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HepG2, 3) Jukat, 4) LNCaP and 5) Raji cell lysate with DFFB antibody. Predicted molecular weight ~39 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate tissue with DFFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HEK293 cells with DFFB antibody; Blue=isotype control, Green= DFFB antibody.