MA5-14955

antibody from Invitrogen Antibodies

Targeting: CBL c-Cbl, CBL2, RNF55

Antibody data

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Validation data

CBL protein structure - MA5-14955 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-Cbl in cell extracts from THP-1 and MCF-7 cells using c-Cbl monoclonal antibody (Product # MA5-14955).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of c-Cbl was achieved by transfecting SH-SY5Y cells with c-Cbl specific siRNA (Silencer® select Product # s2477). Western blot analysis (Fig. a) was performed using whole cell extracts from the c-Cbl knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with c-Cbl Monoclonal Antibody (S.551.7) (Product # MA5-14955, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to c-Cbl.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-c-Cbl Monoclonal Antibody (S.551.7) (Product # MA5-14955) and a 110 kDa band corresponding to c-Cbl was observed. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), BeWo (Lane 2), Daudi (Lane 3) and SH-SY5Y (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).