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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- MA5-15885 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Cbl Monoclonal Antibody (3B12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15885 targets C-CBL in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Rat samples. The MA5-15885 immunogen is purified recombinant fragment of human C-CBL expressed in E. Coli. MA5-15885 detects C-CBL which has a predicted molecular weight of approximately 120kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B12
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C-CBL using a C-CBL monoclonal antibody (Product # MA5-15885) against a human C-CBL (AA: 684-865) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C-CBL using C-CBL monoclonal antibody (Product # MA5-15885) in RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using C-CBL monoclonal antibody (Product # MA5-15885) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL monoclonal antibody (Product # MA5-15885) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL monoclonal antibody (Product # MA5-15885) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using C-CBL monoclonal antibody (Product # MA5-15885) (blue) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using C-CBL monoclonal antibody (Product # MA5-15885) (blue) and negative control (red).
