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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF5998 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse CBL Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse CBL in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22681- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.
Odintsova E, van Niel G, Conjeaud H, Raposo G, Iwamoto R, Mekada E, Berditchevski F
The Journal of biological chemistry 2013 Sep 6;288(36):26323-34
The Journal of biological chemistry 2013 Sep 6;288(36):26323-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CBL by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse CBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5998) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CBL at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
