10918-1-AP

antibody from Proteintech Group

Targeting: CDC27 ANAPC3, APC3, D0S1430E, D17S978E, NUC2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10918-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10918-1-AP, RRID:AB_2075288

Product name

CDC27; APC3 antibody

Antibody type

Polyclonal

Description

CDC27; APC3 antibody (Cat. #10918-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

PSMD14 promotes breast cancer progression by reducing K63-linked ubiquitination on FOXM1 and activating the PI3K/AKT/mTOR pathway.

Liu Y, Wen S, Wang W, Liu Q, Su L, Zhang Y, Bai C, Pu S, Zhang Q, Wang J
International journal of biological macromolecules 2025 Oct;327(Pt 1):147275

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Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway.

Wu HQ, Qin RC, Li WJ, Liu JN, Deng C, Zheng ZH, Zheng JP, Liu Y, Meng YF, Tang C, Tan HM, Duan FF, Tang Y, Xiao F, Lu LW, Dai XY, Ma KY
Acta pharmacologica Sinica 2025 Jul;46(7):2041-2055

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F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression.

Li F, Yu H, Zhang Y, Ma Y, Chen X, Zhang J, Sun L, Guo R, Wu Y, Zheng P, Wang X, Bie P, He F, Zhang L, Xie C, Xiong H
Nature communications 2025 Apr 30;16(1):4060

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ABL1-mediated phosphorylation promotes FOXM1-related tumorigenicity by Increasing FOXM1 stability.

Dong Q, Wang D, Song C, Gong C, Liu Y, Zhou X, Yue J, Hu Y, Liu H, Zhu L, Niu X, Zheng T, Zhang X, Jin J, Wang T, Ju R, Wang C, Jiang Q, Gao T, Jin Y, Li P, Wang Y, Zhang C, Wang GF, Cao C, Liu X
Cell death and differentiation 2024 Oct;31(10):1285-1301

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APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition.

Zhao H, Cheng X, Yan L, Mi F, Wang W, Hu Y, Liu X, Fan Y, Min Q, Wang Y, Zhang W, Wu Q, Zhan Q
Cell communication and signaling : CCS 2024 May 23;22(1):283

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The ubiquitin E3 ligase APC/C(Cdc20) mediates mitotic degradation of OGT.

Meng L, Dong R, Mi W, Qin K, Ouyang K, Sun J, Li J
The Journal of biological chemistry 2024 Jul;300(7):107448

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CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.

Qiu L, Zhou R, Luo Z, Wu J, Jiang H
Frontiers in oncology 2022;12:774458

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EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.

He Y, Gan M, Wang Y, Huang T, Wang J, Han T, Yu B
Cell death & disease 2021 Apr 30;12(5):430

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mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27.

Feng Z, Zhang L, Zhou J, Zhou S, Li L, Guo X, Feng G, Ma Z, Huang W, Huang F
Oncotarget 2017 Jan 24;8(4):6304-6318

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 10918-1-AP(CDC27; APC3 antibody) at dilution of 1:600

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CDC27; APC3 (IP:10918-1-AP, 3ug; Detection:10918-1-AP 1:600) with K-562 cells lysate 11000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 10918-1-AP(CDC27; APC3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue