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Learn10918-1-AP
antibody from Invitrogen Antibodies
Targeting: CDC27
ANAPC3, APC3, D0S1430E, D17S978E, NUC2
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 10918-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDC27/APC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were intracellularly stained with 0.2 µg Anti-Human CDC27; APC3 (Product # 10918-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDC27; APC3 (IP:10918-1-AP, 3ug; Detection:10918-1-AP 1:600) with K-562 cells lysate 11000ug.
