100-4166

antibody from Rockland Immunochemicals, Inc.

Targeting: REL c-Rel, I-Rel

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-4166

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-4166, RRID:AB_2179026

Product name

Anti-NFKB cRel (RABBIT) Antibody - 100-4166

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Targeting activation of specific NF-κB subunits prevents stress-dependent atherothrombotic gene expression.

Djuric Z, Kashif M, Fleming T, Muhammad S, Piel D, von Bauer R, Bea F, Herzig S, Zeier M, Pizzi M, Isermann B, Hecker M, Schwaninger M, Bierhaus A, Nawroth PP
Molecular medicine (Cambridge, Mass.) 2012 Dec 20;18(1):1375-86

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.

Validation comment

Western Blot