600-401-429

antibody from Rockland Immunochemicals, Inc.

Targeting: GSK3A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-429

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-429, RRID:AB_2263781

Product name

Anti-GSK3 alpha pS21 (RABBIT) Antibody - 600-401-429

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts.  Cells were serum starved for 24 h.   Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.  Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3).  Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1,000 dilution of anti-GSK3A antibody.  Signal was detected using standard techniques.  Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada

Validation comment

Western Blot