600-401-444

antibody from Rockland Immunochemicals, Inc.

Targeting: GSK3A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-444

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-444, RRID:AB_2114922

Product name

Anti-GSK3 alpha (RABBIT) Antibody - 600-401-444

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 µg of HEK293 whole cell lysate (lane 1). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO in PBS. Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot