MAB10262

antibody from Novus Biologicals

Targeting: IFNGR1 CD119, IFNGR

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10262

Provider

Novus Biologicals

Product name

Rat Monoclonal IFN-gamma R1/CD119 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma R1/CD119 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) IFN-alpha/beta R1, rmIFN-gamma R2, recombinant human (rh) IFN-gamma R1, rhIL-20 R alpha , rhIL-20 R beta , rmIL-20 R alpha , rhIL-22BP or rmIL-22BP is observed.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production.

Wong KL, Lew FC, MacAry PA, Kemeny DM
European journal of immunology 2008 Aug;38(8):2251-62

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma R1/CD119 Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262). The ND₅₀ is typically 4-20 ng/mL.