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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB10262 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IFN-gamma R1/CD119 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma R1/CD119 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) IFN-alpha/beta R1, rmIFN-gamma R2, recombinant human (rh) IFN-gamma R1, rhIL-20 R alpha , rhIL-20 R beta , rmIL-20 R alpha , rhIL-22BP or rmIL-22BP is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_034641- Isotype
- IgG
- Antibody clone number
- 170911
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production.
Wong KL, Lew FC, MacAry PA, Kemeny DM
European journal of immunology 2008 Aug;38(8):2251-62
European journal of immunology 2008 Aug;38(8):2251-62
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma R1/CD119 Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262). The ND50 is typically 4-20 ng/mL.
