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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1026 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IFN-gamma R1/CD119 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IFN-gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) IFN-gamma RI, recombinant mouse (rm) IFN-alpha/beta R2, rmIFN-gamma R2, rhIL-10 R beta , and rhIL-20 R alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q91Y85- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma R1/CD119 Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1026). The ND50 is typically 0.2-1 µg/mL.
