R35874

antibody from NSJ Bioreagents

Targeting: IFNGR1 CD119, IFNGR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R35874

Provider

NSJ Bioreagents

Product name

IFNGR1 Antibody / CD119

Antibody type

Polyclonal

Description

This highly specific IFNGR1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the IFNGR1 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HepG2 lysate with IFNGR1 antibody at 0.3ug/ml. Predicted molecular weight: ~54/70kDa (unmodified/glycosylated).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HepG2 lysate with IFNGR1 antibody at 0.3ug/ml. Predicted molecular weight: ~54 kDa (unmodified), 80-100 kDa (glycosylated).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human spleen tissue lysate with IFNGR1 antibody at 2ug/ml. Predicted molecular weight: ~54 kDa (unmodified), 80-100 kDa (glycosylated).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human A) K562, B) Caco-2 and 3) HepG2 cell lysate with IFNGR1 antibody at 1ug/ml. Predicted molecular weight: ~54 kDa (unmodified), 80-100 kDa (glycosylated).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human ThP1 cells with IFNGR1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human Caco-2 cells with IFNGR1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung tissue (left) and negative control without primary (right) with IFNGR1 antibody at 4ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human K562 cells with IFNGR1 antibody (blue) at 10ug/ml and naive goat Ig (black).