Explore
Validate
Learn
Western blot
Immunocytochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFNGR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant human IFN-gamma R2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IFNGR1 in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in IFNGR1 polyclonal antibody (Product # PA5-47866) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IFNGR1 in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in IFNGR1 polyclonal antibody (Product # PA5-47866) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IFN- gamma R1/CD119 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-humanIFN- gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47866) at 15 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IFNGR1 Antibody by (Product # PA5-47866) showing the decrease in signal with increasing concentrations of IFNGR1 Antibody. Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IFNGR1 in HeLa human cervical epithelial carcinoma cell line. Samples were incubated in IFNGR1 polyclonal antibody (Product # PA5-47866). Recombinant Human IFN‚γ reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‚γ (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-HumanIFN‚γ R1/CD119 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2 µg/mL.
