13-1199-80

antibody from Invitrogen Antibodies

Targeting: IFNGR1 CD119, IFNGR

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13-1199-80

Provider

Invitrogen Antibodies

Product name

CD119 (IFN gamma Receptor 1) Monoclonal Antibody (GIR-208 (GIR 208)), Biotin, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody reacts with human CD119 also known as the IFN gamma receptor 1 (alpha chain). IFN gamma R1 is found in a complex with IFN gamma receptor beta and recognizes with high affinity IFN gamma. Expression is ubiquitous but low, although some cell types, such as monocytes express higher levels.

Conjugate

Biotin

Antibody clone number

GIR-208 (GIR 208)

Concentration

0.5 mg/mL

References

Submitted references

Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models.

Kemper K, Gielen E, Boross P, Houtkamp M, Plantinga TS, de Poot SA, Burm SM, Janmaat ML, Koopman LA, van den Brink EN, Rademaker R, Verzijl D, Engelberts PJ, Satijn D, Sasser AK, Breij EC
Life science alliance 2022 Nov;5(11)

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Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency.

Hahn K, Pollmann L, Nowak J, Nguyen AHH, Haake K, Neehus AL, Waqas SFH, Pessler F, Baumann U, Hetzel M, Casanova JL, Schulz A, Bustamante J, Ackermann M, Lachmann N
Molecular therapy. Methods & clinical development 2020 Jun 12;17:785-795

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STING contributes to antiglioma immunity via triggering type I IFN signals in the tumor microenvironment.

Ohkuri T, Ghosh A, Kosaka A, Zhu J, Ikeura M, David M, Watkins SC, Sarkar SN, Okada H
Cancer immunology research 2014 Dec;2(12):1199-208

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Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor.

Sheehan KC, Calderon J, Schreiber RD
Journal of immunology (Baltimore, Md. : 1950) 1988 Jun 15;140(12):4231-7

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (blue histogram) or 0.125 µg of Anti-Human CD119 (IFN gamma Receptor 1) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.

Conjugate

Biotin

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Lentiviral Vector Design and Evaluation of Transgene Expression (A and B) Schematic overview of the third-generation SIN lentiviral vectors encoding (A) IFNGR1 cDNA coupled to a GFP reporter via an internal ribosomal entry side (IRES) and (B) a control vector encoding only for the GFP reporter. Transgene expression is driven by a spleen focus forming virus (SFFV) promoter in both constructs. (C) Flow cytometric analysis of GFP and IFN-gammaR1 (CD119) expression in untransduced and Lv.SFFV.IFNgammaR1.iGFP-transduced K562 cells (human myeloid cell line; gray filled: unstained untransduced cells, black: stained untransduced cells, orange: Lv.SFFV.IFNgammaR1.iGFP-transduced K562 cells).

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Different IFN-gammaR1 Expression Levels in HeLa Cells (A) Representative flow cytometry data showing the results of FACS-purification in low, middle (mid), and high IFN-gammaR1 (CD119) expression in Lv.SFFV.IFNgammaR1.iGFP transduced KO HeLa. (B) IFN-gammaR1 (CD119) expression in different HeLa cell lines (n = 4; mean +- SD, statistical analysis was performed using one-way-ANOVA with Dunnett's multiple comparison post hoc testing. *p

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Vector Analysis in SV40 Fibroblasts derived from an IFN-gammaR1 -/- MSMD Patient (A) Detection of green fluorescent protein (GFP) expression and morphology of Lv.SFFV.IFNgammaR1.iGFP-transduced SV40-immortalized fibroblasts from an IFN-gammaR1 -/- -MSMD patient by fluorescence microscopy. Untransduced SV40-immortalized fibroblasts from a healthy donor shown as control (scale bar, 100 muM). (B) Flow cytometric analysis of IFN-gammaR1 (CD119) expression in untransduced IFN-gammaR1 -/- -MSMD SV40-immortalized fibroblasts (gray filled), healthy SV40 fibroblasts (orange), and Lv.SFFV.IFNgammaR1.iGFP-transduced IFN-gammaR1 -/- -MSMD SV40 fibroblasts (blue). (C) Western blot analysis of pSTAT1 upon stimulation with 25 ng/mL hIFN-gamma in untransduced SV40-immortalized fibroblasts from a healthy control, untransduced, and Lv.SFFV.IFNgammaR1.iGFP-transduced SV40-immortalized fibroblasts from an IFN-gammaR1 -/- -MSMD patient with alpha-tubulin as loading control. (D) DeltaMean fluorescence intensity (MFI; corrected to isotype) of IFN-gammaR1 (CD119) expression in corrected and untransduced patient fibroblasts (n = 8 for untransduced cells, n = 4 for transduced cells, mean +- SD; statistical analysis was performed using unpaired t test, *p

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Fas and IFNGR1 expression contribute to the induction of T cell-mediated cytotoxicity by DuoBody-CD3x5T4. (A) 5T4 and Fas expression on MDA-MB-231 parental and Fas KO cells was measured by quantitative flow cytometry with or without prior overnight incubation with IFNgamma (100 ng/ml). (B, C, D, E) MDA-MB-231 parental and Fas KO cells were incubated with purified CD4 + (B, D) or CD8 + (C, E) T cells (E:T ratio = 4:1, n = 5 donors) and DuoBody-CD3x5T4 for 72 h. T-cell activation (B, C) and T cell-mediated cytotoxicity (D, E) were analyzed. (B, C) Activation of T cells from five donors at 1 mug/ml DuoBody-CD3x5T4 (* P

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. DuoBody-CD3x5T4 can induce bystander kill which is dependent on IFNGR1 expression. (A) Parental and 5T4 KO MDA-MB-231 tumor cells were mixed in different ratios, as indicated, and incubated with purified T cells (E:T ratio = 4:1, n = 2 donors) and DuoBody-CD3x5T4 for 72 h. T cell-mediated cytotoxicity of 5T4 + (left panel) and 5T4 - (right panel) tumor cells was determined by flow cytometry, showing a representative donor of three donors tested. (B, C, D) Parental (5T4 + ) MDA-MB-231 tumor cells were cocultured with purified T cells (E:T = 4:1, n = 2 donors) and incubated with 10 mug/ml DuoBody-CD3x5T4 or bsIgG1-CD3xctrl for 72 h. As a positive control, T cells were incubated with anti-CD3/CD28 beads (but without tumor cells) for 72 h. (B) The supernatant (either with or without T cells) was transferred to MDA-MB-231 5T4 KO cells and incubated for 72 h (B). As negative control, MDA-MB-231 5T4 KO cells were incubated with fresh T cells and indicated antibodies for 72 h. (C, D) T cell-mediated cytotoxicity (C) and Fas expression (D) of MDA-MB-231 5T4 KO cells were determined by flow cytometry. A representative donor of two donors tested is shown. (E) 5T4, Fas, IFNGR1, and PD-L1 expression on MDA-MB-231 parental, 5T4/Fas KO, and 5T4/IFNGR1 KO cells was measured by flow cytometry with or without prior overnight incubation with IFNgamma (100 ng/ml). (F, G, H, I) Parental and 5T4/Fas KO or 5T4/IFNGR1 MDA-MB-231 tumor cells were mixed in different ratios, as indicated, and

Conjugate

Biotin