Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF673 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-gamma R1/CD119 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects IFN-gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human IFN-gamma R2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15260
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor.
Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation 2009 Mar 17;119(10):1424-32
Circulation 2009 Mar 17;119(10):1424-32
Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor.
Saidi RF, Williams F, Silberberg B, Mittal VK, ReMine SG, Jacobs MJ
Surgery 2006 Jun;139(6):743-8
Surgery 2006 Jun;139(6):743-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma R1/CD119 in Human PBMCs. IFN-gamma R1/CD119 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673). The ND50 is typically 0.5-2 µg/mL.