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- Antibody Data
- Antigen structure
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- Product number
- M420AB - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 alpha Monoclonal Antibody (ILA8-H12), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M420AB targets IL-1 alpha in ELISA applications and shows reactivity with Human samples. The M420AB immunogen is recombinant human IL-1 alpha. M420AB detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa. The M420AB IL1a antibody (biotinylated conjugate of clone B133.5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M700A (clone 2G1). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M420AB (clone ILA8-H12) and antibody M421A (clone ILA9-H18) have successfully been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- ILA8-H12
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL-1 alpha was performed using a Human IL-1 alpha Colorimetric ELISA Kit (Product # EH2IL1A) by loading 50 µL per well of Human IL-1 alpha Recombinant Protein (Product # SIL1A) in dodecuplicate at 400, 160, 64, 25.6, 10.25 and 0 pg/mL across a 3 µg/mL mouse anti-human IL-1 alpha (Product # M421A) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, then incubated with 50 µL per well of a biotinylated-mouse anti-human IL-1 alpha (Product # M420AB) in dodecuplicate at 0.15 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:10 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
