IC200F

antibody from Novus Biologicals

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC200F

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-1 alpha/IL-1F1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 alpha/IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta , -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.

Ong SM, Hadadi E, Dang TM, Yeap WH, Tan CT, Ng TP, Larbi A, Wong SC
Cell death & disease 2018 Feb 15;9(3):266

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-1 alpha/IL-1F1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes (A) treated with LPS were stained with Mouse Anti-Human IL-1 alpha/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002F). (B) Inhibition of Mouse Anti-Human IL-1 alpha/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) staining by the addition of excess Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.