Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF680 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects porcine IL-1 alpha/IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human IL-1 alpha is observed and 10% cross-reactivity with recombinant mouse IL-1 alpha and recombinant rat IL-1 alpha is observed.
- Reactivity
- Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
Induction of interleukin-1 in articular cartilage by explantation and cutting.
Laussmann MA, Passante E, Hellwig CT, Tomiczek B, Flanagan L, Prehn JH, Huber HJ, Rehm M
The Journal of biological chemistry 2012 Apr 27;287(18):14402-11
The Journal of biological chemistry 2012 Apr 27;287(18):14402-11
Induction of interleukin-1 in articular cartilage by explantation and cutting.
Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
Arthritis and rheumatism 2004 Aug;50(8):2539-46
Arthritis and rheumatism 2004 Aug;50(8):2539-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutral-ization by Porcine IL-1 alpha/IL-1F1 Antibody. Recombinant Porcine IL-1 alpha/IL-1F1 (Catalog # 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 alpha/IL-1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND50 is typically 0.006-0.012 µg/mL in the presence of concanavalin A (1.25 µg/mL).