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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- ELISA [1]
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- Product number
- GTX15754 - Provider product page
- Provider
- GeneTex
- Product name
- IL1 alpha antibody [ILA9-H18]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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- GeneTex (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL1 alpha protein in dodecuplicate at 400, 160, 64, 25.6, 10.25 and 0 pg/ml (50ul per well) using GTX15754 as capture antibody (3ug/ml) and GTX15753 as capture antibody (0.15ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB Substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
